NKI Seminar
17-01-2020 11:00 Piet Borst Auditorium, Netherlands Cancer Institute

International seminar by Gaelle Legube

Gaelle Legube
CBI Toulouse, CNRS, Toulouse University, Toulouse, France
 
Chromosome and chromatin dynamics at DNA Double Strand breaks

 
Friday, January 17, 2020, 11.00 hours
Piet Borst Auditorium, Netherlands Cancer Institute
 
Host: Jacqueline Jacobs
E-mail: j.jacobs@nki.nl

Gaelle Legube
Over the past few years it has become evident that chromatin, that packages DNA in eukaryotes nuclei and that is encoding epigenetic information, is the real substrate for all DNA related processes and plays a decisive role in DNA repair. Repair into this chromatin context raises several questions that we aim to address in the lab. Among the types of damage that can challenge the DNA molecule, DNA Double Strands Breaks (DSBs) are the most deleterious since they can lead to various mutations and chromosomes rearrangements. This type of damage can arise during development as part of programmed processes, but also due to various environmental stress. Over the past few years it has become evident that chromatin, that packages DNA in eukaryotes nuclei and that is encoding epigenetic information, is the real substrate for all DNA related processes and plays a decisive role in DNA repair. Repair of DSB into the chromatin context raises several questions that we aim to address in the lab. Using an experimental system we recently developed (called DIvA for DSB Inducible via AsiSI), that allows the induction of multiple sequence-specific DSBs widespread across the genome, we investigate several uncovered aspects of the relationship between chromatin and DSB repair.